KMID : 0364820100460010093
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Korean Journal of Microbiology 2010 Volume.46 No. 1 p.93 ~ p.95
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Dye Removal by Phlebia tremellosa and Lignin Degrading Enzyme Transformants
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Kum Hyun-Woo
Lee Sung-Sook You Seon-Hwa Choi Hyung-Tae
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Abstract
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White rot fungi which have lignin degrading enzymes show high degrading activity to diverse recalcitrant compounds such as polycyclic aromatic compounds, dyes, explosives and endocrine disrupting chemicals. We have examined decolorizing activity of dyes by Phlebia tremellosa and two transformants which had genetically transformed using laccase or manganese peroxidase (MnP) gene. In case of methyl green, wild type strain showed 50% decolorization while laccase transformant (TF2-1) and MnP transformant (T5) showed more than 90% decolorization on day 3. Remazol
brilliant blue R(RBBR) was decolorized up to 85% by two transformants while the wild type showed 67% decolorization on day 3. Transformants TF2-1 and T5 both showed increased laccase and MnP activity respectively during the whole growing phase.
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KEYWORD
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dye decolorization, genetic transformant, methyl green, P. tremellosa, remazol brilliant blue R
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